Ethanol Extract of Alcea Rosea Flowers Has a Suppressive Effect on Adipogenesis Under In Vitro Conditions

Ebru Demir; Ceyda Okudu; Özlem Ağırel; Hüseyin Servi

doi:10.5281/zenodo.14569158

Authors

Ebru Demir Haliç University https://orcid.org/0000-0001-9470-214X
Ceyda Okudu Haliç Üniversitesi
Özlem Ağırel Haliç University https://orcid.org/0000-0002-2776-919X
Hüseyin Servi İstanbul Yeni Yuzyil University https://orcid.org/0000-0002-4683-855X

DOI:

https://doi.org/10.5281/zenodo.14569158

Keywords:

Adipogenesis, Alcea rosea, Obesity

Abstract

Objective: Obesity is a disease that causes serious health complications and is seen all over the world and there is a need to develop new long-term and effective alternative anti-obesity strategies. Natural and herbal products are widely used in treating obesity due to their various biological activities, including anti-inflammatory, antioxidant, and anti-obesity effects. It was aimed to investigate the effects of Alcea rosea flower on lipid accumulation and PPARγ and CERBβ biomarkers that have a key role in adipogenesis in this study.

Material and Methods: To mimic adipogenesis in vitro, human precursor adipocyte cells were used and grown in a preadipocyte growth medium at 37°C in a 5% CO₂ incubator. The grown cells were seeded at 10⁵ cells/well, then divided into 4 groups, and different doses (50 μg 100 μg 150 μg and 200 μg) of Alcea rosea ethanol extract (ARE) and differentiation medium were applied to each group. At the end of the 15^th day, Oil O Red staining was performed to measure lipid accumulation. PPARγ and C/EBPβ gene expressions were determined by the RT-PCR method.

Results: Lipid accumulation was significantly suppressed (p<0,05) at all ARE doses. In addition, ARE suppressed PPARγ gene expression at all doses, but the suppression was statistically significant only at the 100 μg applied level (p=0,05). In the analysis of the C/EBPβ gene, suppression was observed at all doses, but the suppression was found to be statistically significant only at the 50 μg (p=0,04) and 100 μg (p=0,04) doses.

Discussion and Conclusion: As a result, it was determined that the applied ARE concentrations suppressed adipogenesis and had anti-obesity properties, but it is recommended that further studies support the study findings.

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