Rutaceae (Sedefotugiller) Ailesine Ait Bazı Haplophyllum Türlerinin Sitotoksik Etkileri Üzerine Bir Çalışma: H. Gilesii, H. Tuberculatum, H. Myrtifolium, H. Megalanthum, H. Buxbaumii, H. Acutifolium ve H. Vulcanicum

Mustafa Orhan Tunçel; Mehmet Ramat; Kadir Eği

doi:10.5281/zenodo.17251664

Authors

Mustafa Orhan Tunçel İnönü University https://orcid.org/0000-0002-8999-2223
Mehmet Ramat Harran University https://orcid.org/0000-0003-4922-6708
Kadir Eği Harran University

DOI:

https://doi.org/10.5281/zenodo.17251664

Keywords:

Phytochemical compounds, Haplophyllum species, Cancer cell lines, Selective anticancer activity, Cytotoxicity

Abstract

Objective: To summarize the phytochemical diversity and potential cytotoxic/antiproliferative effects of the genus Haplophyllum (H. gilesii, H. tuberculatum, H. myrtifolium, H. megalanthum, H. buxbaumii, H. acutifolium, H. vulcanicum) of the Rutaceae family on cancer cell lines in the light of the current literature and to identify research gaps.

Material and Methods: This study is a review. A structured search was conducted in international databases (e.g., PubMed, Scopus, Web of Science) using relevant species names and keywords such as “Haplophyllum,” “phytochemistry,” “coumarin/alkaloid/lignan/flavonoid,” “cytotoxicity,” “antiproliferative,” “apoptosis,” and “IC50.” The search focused on original research, isolation/chemical characterization, and in vitro biological activity studies.

Results: Haplophyllum species are rich in coumarins, alkaloids, lignans, and flavonoids; these classes have been shown to be associated with antiproliferative and apoptotic responses in tests such as MTT, WST-1, and brine shrimp. H. acutifolium and H. buxbaumii, in particular, stand out for their selective cytotoxicity, while low IC₅₀ values for some compounds isolated from H. megalanthum indicate high anticancer potential. Their activity profiles vary in accordance with the phytochemical diversity of the species.

Discussion and Conclusion: Literature indicates that Haplophyllum species offer strong candidates for pharmaceutical research. Their selective activity and low IC₅₀ values are promising for drug discovery. However, standardized extraction/experimental designs, mechanistic validation (target/pro-apoptotic pathways), and in vivo/ADME toxicity data are limited. Future studies should focus on target identification and structure-activity analysis at the pure compound level, with sustainable sampling while considering the conservation of biodiversity.

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