Rutaceae (Sedefotugiller) Ailesine Ait Bazı Haplophyllum Türlerinin Sitotoksik Etkileri Üzerine Bir Çalışma: H. Gilesii, H. Tuberculatum, H. Myrtifolium, H. Megalanthum, H. Buxbaumii, H. Acutifolium ve H. Vulcanicum

Mustafa Orhan Tunçel; Mehmet Ramat; Kadir Eği

doi:10.5281/zenodo.17251664

Yazarlar

Mustafa Orhan Tunçel İnönü Universitesi https://orcid.org/0000-0002-8999-2223
Mehmet Ramat Harran Üniversitesi https://orcid.org/0000-0003-4922-6708
Kadir Eği Harran University

DOI:

https://doi.org/10.5281/zenodo.17251664

Anahtar Kelimeler:

Fitokimyasal bileşikler, Haplophyllum türleri, Kanser hücre hatları, Seçici antikanser etki, Sitotoksisite

Özet

Amaç: Rutaceae familyasına ait Haplophyllum cinsinin (H. gilesii, H. tuberculatum, H. myrtifolium, H. megalanthum, H. buxbaumii, H. acutifolium, H. vulcanicum) fitokimyasal çeşitliliğini ve kanser hücre hatları üzerindeki potansiyel sitotoksik/antiproliferatif etkilerini mevcut literatür ışığında özetlemek ve araştırma boşluklarını belirlemek.

Materyal ve Metot: Bu derleme çalışmasında gili tür adları ve “Haplophyllum”, “phytochemistry”, “coumarin/alkaloid/lignan/flavonoid”, “cytotoxicity”, “antiproliferative”, “apoptosis”, “IC50” anahtar kelimeleri kullanılarak uluslararası veri tabanlarında (ör. PubMed, Scopus, Web of Science) yapılandırılmış arama yapılmış; özgün araştırma, izolasyon/kimyasal karakterizasyon ve in vitro biyolojik aktivite çalışmalarına odaklanılmıştır.

Bulgular: Haplophyllum türlerinin kumarinler, alkaloitler, lignanlar ve flavonoidlerce zengin olduğu; bu sınıfların MTT, WST-1 ve brine shrimp gibi testlerde antiproliferatif ve apoptotik yanıtlarla ilişkili bulunduğu görülmüştür. Özellikle H. acutifolium ve H. buxbaumii seçici sitotoksisite açısından öne çıkarken, H. megalanthum’dan izole edilen bazı bileşiklerin düşük IC₅₀ değerleri yüksek antikanser potansiyeli işaret etmektedir. Etki profilleri, türlerin fitokimyasal çeşitliliğiyle uyumlu bir biçimde değişkenlik göstermektedir.

Tartışma ve Sonuç: Literatür, Haplophyllum türlerinin farmasötik araştırmalar için güçlü adaylar sunduğunu göstermektedir. Seçici etki ve düşük IC₅₀ değerleri ilaç keşfi açısından umut vericidir. Bununla birlikte, standardize ekstraksiyon/deney tasarımları, mekanistik doğrulamalar (hedef/pro-apoptotik yolaklar) ve in vivo/ADME-toksisite verileri sınırlıdır. Gelecek çalışmalar, biyolojik çeşitliliğin korunmasını gözeterek sürdürülebilir örneklemeyle, saf bileşik düzeyinde hedef tayini ve yapı-etki ilişkisi analizlerine odaklanmalıdır

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